HPV Genotyping – Human Papillomavirus

HPV 3.5 LCD-Array Kit

Parallel, specific identification of 32 HPV types (Human Papillomavirus genotyping). HPV types 06 ,11 ,16 ,18 ,31 ,33 ,35 ,39, 42 ,44 ,45 ,51 ,52 ,53 ,54 ,56, 58 ,59 ,61 ,62 ,66 ,67 ,68 ,70, 72 ,73 ,81 ,82 ,83 ,84 ,90 and 91 are identified with a single hybridisation of two PCR amplifications into one array field. Due to small amplicon sizes and the combination of two robust PCR systems, the assay is well suited for the analysis of formalin-fixed, paraffin-embedded (FFPE) tissue samples.

See graphic below for HPV type risk classification according to WHO IARC Monograph 100B (2011).

PCR coupled DNA macroarray (reverse hybridisation). Biotinylated PCR products are generated from nucleic acid extracts of sample material containing human papillomavirus DNA. The labeled amplicons are hybridized to species-specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers which can be addressed individually

The assay is based on two PCR reactions which are combined prior to hybridization into one array field. Both primer mixes are directed against highly conserved motifs within the viral L1 gene. Primer mix A generates fragments of approx. 450 bp in length (HPV type dependent), while primer mix B generates amplicon sizes of approx. 165 bp (HPV type dependent). Combining both independent single PCRs prior to hybridisation into one array field ensures the parallel and robust detection of all 32 HPV types at 5 target copies per reaction for HPV types 16, 18 and 31 and at 50 target copies for all other HPV types. The assay is also well suited for the analysis of formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Brochure DNA Array HPV Typing

LCD Array Kit

HPV 3.5

Parallel identification of 32 HPV types (Human Papillomavirus Genotyping)

Order codes:

32 Tests   [ V-100-04 ]

96 Tests   [ V-100-12 ]

  Brochure   MSDS

Spotting pattern DNA Array HPV 3.5

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Analysis Screen Shots

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Related Literature

  • Global Improvement in Genotyping of Human Papillomavirus DNA: the 2011 HPV LabNet International Proficiency Study (2014). Eklund C., Forslund O., Wallin K.-L. and Dillner J.  J. Clin. Microbiol., 52(2): 449-459  LINK:   PDF at JCM
  • Shift in prevalence of HPV types in cervical cytology specimens in the era of HPV vaccination (2016). Fischer S., Bettstetter M., Becher A., Lessel M., Bank C., Krams M. Becker I., Hartmann A., Jagla W. and Gaumann A.  Oncology Letters, 12(1): 601-610  LINK:   PDF at NCBI
  • Comparison of molecular multiplex assays for the detection of human papillomaviruses from clinical gynecological tissue samples (2014). Kapisch I., Silling S., Brockmann M., Schildgen O., Tillmann R.-L. and Schildgen V.  Future Virology 9(1): 21-26  LINK:   futuremedicine
  • HPV16 DNA status is a strong prognosticator of loco-regional control after postoperative radiochemotherapy of locally advanced oropharyngeal carcinoma: Results from a multicentre explorative study of the German Cancer Consortium Radiation Oncology Group (2014). Lohaus F. et al. [for the DKTK-ROG]  Radiotherapy and Oncology , 113(3): 317-323.  LINK:   PDF at sciencedirect
  • Cervical biopsies and cytological smears – A comparison of sample materials in HPV diagnostics (2013) Webersinke C., Doppler S., Roithmeier F., Stummvoll W. and Silye R.  Journal of Clinical Virology , 56 (2013) 69– 71  LINK:   PDF at JCV
  • Comparison of In-House Multiplex Real Time PCR, Diagcor GenoFlow HPV Array Test and INNO-LiPA HPV Genotyping Extra Assays with LCD- Array Kit for Human Papillomavirus Genotyping in Cervical Liquid Based Cytology Specimens and Genital Lesions in Tehran, Iran (2016).  Sohrabi A., Farzami M., Samiee S.M. and Babaei M.  Clinical Laboratory , 62(4): 615-619.  LINK:   clinical-laboratory
  • HPV DNA and Pap smear test results in cases with and without cervical pathology (2012). Özalp S.S., Us T., Arslan E., Öge T. and Kaşifoğlu N.  J. Turkish-German Gynecol. Assoc. , 13: 8-14. LINK:   NCBI
  • Inter-laboratory validation of PCR-based HPV detection in pathology specimens (2007).  Petersen I., Schewe C., Schlüns K., Dietel M., Speich N., Schmitt C., Bollmann M., Sotlar K., Bültmann B., Dours-Zimmermann M.T., Padberg B. and Zimmermann D.R.  Virchows Arch. , 451: 701–716. LINK:   link.springer.com
  • Polymerase Chain Reaction Study of Anogenital Condylomas for High Risk HPV Types (2009) Nassiri S., Sarlak M., Posh F.B.Z., Samiee S.M., Rahimi F. and Qaisari M.  Iranian Journal of Dermatology, Vol 12(48): 52-55. LINK:   iranjd.ir
  • Molecular HPV Typing as a Diagnostic Tool to Discriminate Primary From Metastatic Squamous Cell Carcinoma of the Lung (2009). Weichert W., Schewe C., Denkert C., Morawietz L., Dietel M. and Petersen I.  Am. J. Surg. Pathol. , 33: 513–520.LINK:  NCBI
  • HPV-DNA testing and Ki-67 immunocytochemistry in liquid based cervical cytology in prostitute women (2006). Atik E., Otlu B., Çetin M., Tok D. and Durmaz R. Turkish Journal of Pathology, 22(3): 167-173.LINK:   PDF at turkjpath.org

HPV Plus 2.0 LCD-Array Kit

Identification of 9 additional HPV types (Type 02, 05, 07, 12, 13, 27, 32, 55 and 57) by using PCR products of primer mix A and B from the HPV 3.5 kit for hybridisations against HPV-Plus 2.0 arrays. No extra PCR required. The HPV-Plus 2.0 LCD-Array Kits are available as supplemental products only. The kits contain all detections chemicals required for hybridisation and staining, but no primer mixes.

See LCD-Array Kit HPV 3.5

See LCD-Array Kit HPV 3.5

LCD Array Kit

HPV Plus 2.0

Identification of 9 additional HPV types

Order codes:

32 Tests   [ V-125-04 ]

96 Tests   [ V-125-12 ]

Accessory Product to HPV 3.5 Kit

No additional PCR required

Spotting pattern DNA Array HPV Plus 2.0

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Analysis Screen Shots

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