FUNGI 2.1 LCD-Array Kit
Fast, parallel and sensitive screening for the presence of DNA from clinically relevant fungal species, genera and complexes with a single hybridisation assay. The detection range covers 11 species originally belonging to the genus Candida as well as the Aspergillus niger complex, A. fumigatus complex, A. flavus, A. versicolor and A. terreus and different species from the genera Emericella, Mucor, Rhizopus, Rhizomucor, Paecilomyces, Cryptococcus, Scedosporium and Absidia.
Due to a very robust universal PCR targeting the ITS2 region within the fungal genomes and the small amplicon sizes (~250-300 bp) the assay is well suited for the analysis of formalin-fixed, paraffin-embedded (FFPE) tissue samples.
PCR coupled DNA macroarray (reverse hybridisation). Biotinylated PCR products are generated from nucleic acid extracts of sample material containing fungal DNA. The labeled amplicons are hybridized to species-specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers which can be addressed individually.
The primer mix is directed against highly conserved motifs flanking the internal transcribed spacer region (ITS2) of fungal genomes. Specific capture probes immobilized as duplicates permit the simultaneous identification of 24 fungal pathogens on the species or genus level or as members of a specific complex. Due to the small amplicon sizes (~250-300 bp) the assay is well suited for the analysis of FFPE tissue sections. The detection limit of the assay equals 10 genome equivalents (GE) per reaction. With respect to the ongoing changes in fungal systematics and the uncertainty of several data base entries, a detailed description of probe specificities is provided within the kit manual.
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Analysis Screen Shots
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- Diagnosis of chronic disseminated candidosis from liver biopsies by a novel PCR in patients with haematological malignancies (2012). Fleischhacker M., Schulz S., Jöhrens K., von Lilienfeld-Toal M., Held T., Fietze E., Schewe C., Petersen I. and Ruhnke M. Clin. Microbiol. Infect. 2012; 18: 1010–1016. LINK: PDF at onlinelibrary.wiley.com
- Molecular identification of fungal pathogens in nodular skin lesions of cats (2015). Bernhardt A., von Bomhard W., Antweiler E. and Tintelnot K. Med. Mycol. 53 (2): 132-144. LINK: academic.oup.com