LCD-Array kit products for bacterial pathogens
LCD-Array kit products for DNA/PCR based identification of bacterial pathogens: Mycobacteria, MTB, MTBC, MOTT, Periodontitis and more

Mycobacteria Identification

MYCO-Direct 1.7 LCD-Array Kit

Reliable and sensitive identification of members of the Mycobacterium tuberculosis complex (MTBC), Mycobacterium avium-intracellulare complex (MAC) and 10 mycobacteria other than tuberculosis (MOTT). Three aditional capture probes (Mycobacteria I-III) directed against conserved motifs within the ITS region of mycobacteria can furthermore indicate the presence of DNA from other mycobacteria without providing species information. Due to small amplicon sizes and the combination of two robust PCR systems, the assay is well suited for the analysis of formalin-fixed, paraffin-embedded (FFPE) tissue samples.

The specific detection range can be broadened to more mycobacterial species (e.g. M. fortuitum, M. gastri, M. leprae and others) by simply hybridising the PCR products generated with primer mix A (ITS) to MYCO-Plus 2.0 arrays without the requirement for an additional PCR. (see below for more details).

PCR coupled DNA macroarray (reverse hybridisation). Biotinylated PCR products are generated from nucleic acid extracts of sample material containing mycobacterial DNA. The labeled amplicons are hybridized to species-specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers which can be addressed individually.

The assay is based on two PCR reactions which are combined prior to hybridization into one array field. Primer mix A directed against the internal transcribed spacer (ITS) region of mycobacteria and primer mix B directed against the insertion element IS6110 exclusively found in members of the MTBC. Both PCRs generate amplicons of less than 350 bp length (Mix A, ITS: ~200-300 bp, Mix B, IS6110: 127 bp) and allow for the detection of 50 genome equivalents (GE) per reaction for MOTT (ITS) and 1 GE for MTBC (IS6110).

Brochure DNA Array Mycobacteria MTBC

LCD Array Kit

MYCO-Direct 1.7

Identification of Mycobacterium tuberculosis complex (MTBC) & 11 mycobacteria other than tuberculosis (MOTT)

Order codes:

32 Tests   [ B-500-04 ]

96 Tests   [ B-500-12 ]

  Brochure   MSDS

Spotting pattern DNA Array MYCO-Direct 1.7

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Analysis Screen Shots

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Related Literature

  • Comparison of 14 Molecular Assays for Detection of Mycobacterium tuberculosis Complex in Bronchoalveolar Lavage Fluid (2013). Akkerman O.W., van der Werf T.S., de Boer M., de Beer J.L., Rahim Z., Rossen J.W.A., van Soolingen D., Kerstjens H.A.M. and van der Zanden A.G.M. J. Clin. Microbiol.,51(11),3505-3511   LINK:   PDF at jcm.asm.org
  • Identification of HPV Types and Mycobacterium Tuberculosis Complex in Historical Long-Term Preserved Formalin Fixed Tissues in Different Human Organs (2017). Huehns M., Erbersdobler A., Obliers A. and Roepenack P. PLoS One, 12(1):e0170353   LINK:   PDF at plos.org

MYCO-Plus 2.0 LCD-Array Kit

Identification of 9 additional mycobacteria on the species level by using PCR products of primer mix A (ITS) from the MYCO-Direct 1.7 kit for hybridisations against MYCO-Plus 2.0 arrays. No extra PCR required. The MYCO-Plus LCD-Array Kits are available as supplemental products only. The kits contain all detections chemicals required for hybridisation and staining, but no primer mixes.

See MYCO-Direct 1.7

See MYCO-Direct 1.7 (PCR with primer mix A directed against mycobacterial ITS region)

LCD Array Kit

MYCO-Plus 2.0

Identification of 9 additional Mycobacteria

Order codes:

32 Tests   [ B-650-04 ]

96 Tests   [ B-650-12 ]

Accessory Product to MYCO-Direct 1.7

No additional PCR required

Spotting pattern DNA Array MYCO-Plus 2.0

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Analysis Screen Shots

Screenshot Analysis LCD-Array MYCO Plus

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