Food Authenticity Testing

DNA based identification of 24 animal species in raw & processed food

MEAT 5.0 LCD-Array Kit

Fast, reliable and sensitive screening for DNA from 24 animal species with a single PCR / hybridisation assay.

The MEAT 5.0 LCD-Array Kit has especially been developed for laboratories working in the field of quality assurance and food authenticity testing. Comprising excellent specificity and detection limits of < 1% even in extensively processed food matrices (Fc-values > 24) it is the perfect tool to identify unwanted, undeclared or intentionally mislabelled adulterations along the entire supply chain.

Due to the very robust universal primer system targeting a small area (125-165 bp) of the vertebrate 16S rRNA the scope of the assay can easily be broadened by hybridising the same PCR product to a second macroarray (MEAT Plus 3.0) covering additional 15 animal species without the requirement for a new PCR (see below).

PCR coupled DNA macroarray (reverse hybridisation). Biotinylated PCR products are generated from nucleic acid extracts of sample material containing animal DNA. The labeled amplicons are hybridized to species-specific capture probes immobilized on the LCD-Chip surface. Following a short wash routine, comprising high stringency, visualization of bound amplicons is mediated by an enzyme-substrate cascade. Each LCD-Chip contains eight identical micro arrays separated in small reaction chambers which can be addressed individually

The assay is based on a single tube PCR amplification using primer pairs directed against a 125-165 bp fragment of the mitochondrial 16S rRNA gene. Due to the multiple copies of the mitochondrial genome per cell, the assay sensitivity is sufficient to robustly detect 0.5 to 1 nuclear genome equivalents per species. Detection limits with respect to relative amounts of wet or dry weight in mixtures containing tissue / DNA from more than one animal species are in the range of 0.1 %. For all details and experimental data about the assay sensitivity and specificity refer to the PDF Reports and/or to the peer review publications listed below.

Brochure Animal Species Identification MEAT

LCD Array Kit

MEAT 5.0

Parallel identification of 24 animal species in raw and processed food matrices

Order codes:

32 Tests   [ A-500-04 ]

96 Tests   [ A-500-12 ]

  Brochure   MSDS

Spotting pattern DNA Array MEAT 5.0

Click pattern to enlarge

Analysis Screen Shots

Click image to enlarge

  1. 1 PCR + 1 ARRAY = 24 ANIMAL SPECIES

  2. UNIVERSAL PCR – 16S rRNA

  3. 8 ARRAYS / CHIP

  4. DETECTS 1 pg or 0.1-0.5% ADULTERANT

  5. RAW & PROCESSED FOOD, F-VAL > 24

  6. MINIMAL INSTRUMENTATION

  7. NON-FLUORESCENT CHEMISTRY

  8. QUICK SOFTWARE ANALYSIS

Specificity

Sensitivity

Animal Species Identification Sensitivity Report

Processed Meat

Animal Species Identification Processed Food

Application Notes

Animal Species Identification Application Notes

Related Literature

  • A DNA macro-array to simultaneously identify 32 meat species in food samples (2016). Cottenet G., Sonnard V., Blancpain C., Ho H.Z., Leong H.L. and Chuah, P.F. Food Control, 67, 135-143   LINKS:   sciencedirect or ResearchGate
  • Validation of a DNA biochip for species identification in food forensic science (2017). Beltramo C., Riina M.V. , Colussi S., Campia V., Maniaci M.G., Biolatti C., Trisorio S., Modesto P., Peletto S. and Acutis P.L. Food Control, 78, 366-373   LINK:   sciencedirect
  • Eurofins Unveils Multiplexed DNA Chip-based Testing Service to Detect Unwanted Animal DNA in Food and Feed (2015). Han A.P. Genomeweb Premium Article, published Jun 16, 2015 at https://www.genomeweb.com   LINKS:   businesswire or  genomeweb
  • A high incidence of species substitution and mislabeling detected in meat products sold in South Africa (2013). Cawthorn D.-M., Steinman H.A. and Hoffman L.C. Food Control, 32, 440-449.   LINK:   PDF at scholar.sun.ac.za
  • Food Forensics: Using DNA-Based Technology for the Detection of Animal Species in Meat Products (2014). Yosef T.A., Al-Julaifi M.Z. and AL-Rizqi A.M. Nature and Science, 12(6), 82-90.   LINK:   PDF at sciencepub.net
  • Biochip technology for the detection of animal species in meat products (2011). Iwobi A.N., Huber I., Hauner G., Miller A. and Busch U. Food Analytical Methods, 4, 389-398.   LINKS:   link.springer or  ResearchGate

 

Broaden the detection range by additional 15 animal species

MEAT Plus 3.0 LCD-Array Kit

Identification of 15 additional animal species by using PCR products already generated with the primer mix MEAT from the MEAT 5.0 kit for hybridisations against MEAT Plus 3.0 arrays. No extra PCR required. The HPV-Plus 2.0 LCD-Array Kits are available as supplemental products without primer mix or as a stand alone product including the MEAT primer mix. Both kits contain all detections chemicals required for hybridisation and staining.

See LCD-Array Kit MEAT 5.0

See LCD-Array Kit MEAT 5.0

Brochure Animal Species Identification MEAT

LCD Array Kit

MEAT Plus 3.0

Identification of additional 15 animal species.

Order codes:

32 Tests   [ A-925-04 ]

96 Tests   [ A-925-12 ]

Accessory Product to MEAT 5.0

No additional PCR required

Spotting pattern DNA Array MEAT Plus 3.0

Click pattern to enlarge

Analysis Screen Shots

Analysis Screenshot MEAT Plus LCD-Array

Click image to enlarge

Animal Control DNA

High molecular weight genomic DNA extracted from fresh muscle tissue. Available for over 24 animal species. Spectrophotometrically quantified, RNAse treated and tested for integrity by electrophoresis, PCR amplification and hybridisation of amplicons against the MEAT 5.0 and the MEAT Plus 3.0 LCD-Arrays. A260/280: ≥ 1.8

Our Animal Control DNAs are delivered in 0.5 x TE Buffer at 100 ng/µl (+/- 2) and are shipped without cooling (express delivery). Each vial contains 25 µl (2.5 µg in total). Vials should be stored at -20°C upon arrival.

For routine use as positve controls we recommend to dilute the stock solution 1:10 by adding 225 µl 0.5 x TE buffer at arrival and storage of the diluted aliquots with 10 ng/µl at -20°C. For sensitivity tests using end dilution rows it is recommended to start from the concentrated stock solution with 100 ng/µl.

Specifications

High molecular weight animal DNA extracted from fresh muscle tissue.

  • 100 ng/µl,  A260/280: ≥ 1.8
  • Treated with RNAse, delivered in 0.5 x TE
  • Species confirmed by sequencing (cytB or coxI)
  • PCR amplificability tested at 100 ng/µl – free of inhibitors
  • Tested negative for 38 other animal species

Order Information

Animal Control DNA: [ ACD-XXX-025]

1 species, 1 vial, 25 µl, 100 ng/µl

Animal Control DNA, Set of 4: [ ACD-100-04]

Choose from list, 4 species, 4 vials, 4 x 25 µl, 100 ng/µl